DLC ( Differential lecocytes count )
DLC ( Differential leukocyte count )
Introduction - इस test मे different types WBC को अलग - अलग count किया जाता है
AIM - किसी भी patient मे
Nutrophilia Or Nitropenia
Eosinophilia Or Eosinipenia
Basophilia Or Basopenia
Monocytosis Or monocytopenia
Lymphocytosis Or Lymphocytopenia
को पता करने के लिए किया जाता है
METHED - SMEAR METHED
Requriment - microscope, glass slide, sperder, methenol fild stain A, methenol fild stain B.
Semple requriment - patient का whole blood.
Principle - different type के wbc के cytoplasm तथा nucleus का part अलग अलग होता है जिसके कारण यह Stain के अलग-अलग Colour को hold करते हैं
Procedure - सबसे पहले proper technique से thin smear तैयार करते हैं और इसे air dry करते हैं इसके बाद air dry smear को methanal से fix करते हैं इसके बाद Smear को fild stain A मे 7 से 8 second तक Stain करते हैं और Water से wash करते हैं इसके बाद fild stain B मे 12 से 15 second तक stain करते हैं इसके बाद water से wash करते हैं इसके बाद fild stain A मे 5 से 8 second तक stain करते हैं और water से wash करते हैं फिर Air dry करते हैं Air Dry करने के बाद microscope के high power lens मे examination करते हैं तथा 100 wbc को count करते हैं
Normal value -
Nutrophili 40 - 70 %
Eosinophil. 01 - 06 %
Basophil. 00 - 01 %
Monocyte 05 - 15 %
Lymphocyte 20 - 40 %
LAL BAHADUR YADAV
( DMLT )
English translet -
DLC (Differential leukocyte count)
Introduction - In this test different types of WBC are counted differently.
AIM - In any patient
Nutrophilia Or Nitropenia
Eosinophilia or Eosinipenia
Basophilia or basopenia
Monocytosis Or Monocytopenia
Lymphocytosis Or Lymphocytopenia
Is used to address
METHED - SMEAR
Requriment - microscope, glass slide, sperder, methenol fild stain A, methenol fild stain B.
Semple requriment - whole blood of the patient.
Principle - The cytoplasm and nucleus part of wbc of different types are different due to which they hold different colors of stain.
Procedure - First prepare the thin smear with proper technique and air dry it, then fix the air dry smear with methanal, after this, stain the Smear from fild stain A for 7 to 8 second and wash with water. After this, fild stain B stain from 12 to 15 second, after this, wash with water, after this, fild stain A, stain from 5 to 8 second and wash with water, then air dry to air dry. After examination in high power lens of microscope and count 100 wbc.
Normal value -
Nutrophili 40 - 70%
Eosinophil. 01 - 06%
Basophil. 00 - 01%
Monocyte 05 - 15%
Lymphocyte 20 - 40%
LAL BAHADUR YADAV
(DMLT)
Nace
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